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. 2015 Apr 13;10(4):e0125504. doi: 10.1371/journal.pone.0125504

Fig 6. Measure of Neuroinflammation.

Fig 6

Neuroinflammation was assessed by measuring the voxel intensity of cerebral glial fibrillary acidic protein (GFAP) expression in cerebral cortex (A) and hippocampal formation (B) in Sprague-Dawley rats and C57BL/6J mice fed 80,000 IU vitamin D (VD) per kilogram of diet and their respective controls, expressed as voxels per volume unit. Cerebral GFAP expression in cortex (C) and hippocampal formation (D) in parathyroid gland ablated (PTX) rats and parathyroid hormone (PTH) infused rats with their controls are also shown. Statistical difference represented by different letters (p<0.05; n = 4–10; one way ANOVA followed by Tukey’s post hoc test). Data is shown as mean ± SEM. Representative 2-dimensional immunofluorescent micrographs of neuroinflammatory GFAP, in extended focus, are shown in the last frame. Each intervention (vitamin D enrichment; PTX; PTH-infusion) and their respective controls are indicated. GFAP is shown in yellow and nuclei in blue. Scale bar indicates 100μm.