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. 2015 Apr 13;10(4):e0123257. doi: 10.1371/journal.pone.0123257

Fig 4. Blocking eNOS uncoupling mitigates TNFα-induced endothelial dysfunction and inflammatory response.

Fig 4

A, TNFα decreases VE-cadherin and eNOS expression in VEC over 48 hours (arrows), rescued by BH4 or peg-SOD. TNFα increases VCAM-1 expression in VEC over 48 hours, mitigated by BH4 or peg-SOD. B, Western blot analysis of VE-cadherin (VE-cad), eNOS, and VCAM-1 protein in same conditions as A, with GAPDH housekeeping protein. C, Quantification of western blot results, normalized to GAPDH and control, n = 3. * indicates p < 0.05 versus control, # indicates p < 0.05 versus TNFα. Scale bar = 20μm. Images representative of three independent experiments.