Fig 3. Changes in nucleotides after exposure of platelets to thrombin.

Platelets (1 x 10⁸) were plated onto Cell-Tak coated 48 well plates, and exposed to either media or thrombin (0.5 U/ml) for 30 min and the samples were extracted to measure (A) NAD, AMP, ADP and ADP by HPLC. (B) The ATP/ADP ratio and (C) energy charge (ATP + 1/2ADP)/(ATP+ADP+AMP) was calculated. Data expressed as mean±SEM from one representative donor, n = 3 replicates per sample. *p<0.05, **p<0.01, different from control.