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. 2015 Apr 13;10(4):e0122678. doi: 10.1371/journal.pone.0122678

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© 2015 Yu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) The matched normal (normal) and cancerous (cancer) tissues from each patients selected randomly were analyzed by RT-PCR using PAR4- and GAPDH- specific primers (n = 4). After the samples were normalized to GAPDH levels, mRNA levels of PAR4 and TFF2 were significantly increased in cancer compared to the normal tissues; (B) Western blot of tissue lysates from four cases of colorectal cancer (cancer) and relevant adjacent non-neoplastic mucosa (normal). The expression of actin was served as a control. The significant up-regulation expression of PAR4 was observed in the cancerous tissues in contrast to their matched normal tissues (n = 4).