Ten-d-old CA4002 plants were first infiltrated with Agrobacterium carrying pYL156-GhBAK1 or vector control (pYL156-GFP), and then inoculated with V. dahliae (isolate King) suspension spores at a concentration of 1 × 106/mL.
(A) Whole plants (top panel) and detached leaves (bottom) are shown at 14 d after V. dahliae infection.
(B) Percentage of plants showing Verticillium wilt phenotype at the indicated days post-infection. The disease ratio was scored using 21 plants per treatment and the assays were repeated three times with similar results.