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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jun 9;73:411–420. doi: 10.1016/j.freeradbiomed.2014.06.002

Figure 1. GRP78 is a target for modification by 4-HNE and 4-ONE in a murine model for early-stage ALD.

Figure 1

Positive immunostaining was observed following western blotting with antibodies directed against 4-HNE-modified proteins (A) and 4-ONE-modified proteins (B). As a reference, immunostaining was also conducted with antibodies directed against the C-terminal KDEL sequence of GRP78 (C) Coomassie blue staining reveals GRP78, which was positively identified utilizing MALDI-MS (D). GRP78 is indicated by the arrow in each figure.