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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jun 9;73:411–420. doi: 10.1016/j.freeradbiomed.2014.06.002

Figure 2. Carbonylation of GRP78 is increased following sustained ethanol consumption.

Figure 2

(A) Carbonylated GRP78 is visualized through biotin hydrazide conjugation, streptavidin pulldown and GRP78 western blotting. While the expression of GRP78 does not change following ethanol consumption, carbonylated protein is increased. (B) Blot densitometry of (A) reveals a statistically significant increase in modified protein.