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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Free Radic Biol Med. 2014 Jun 9;73:411–420. doi: 10.1016/j.freeradbiomed.2014.06.002

Figure 3. Adduction by 4-HNE and 4-ONE leads to proteins-protein cross-linking.

Figure 3

A.) Western blotting of purified GRP78 following adduction with either 4-HNE or 4-ONE. Adduction induces high molecular weight protein-protein cross-linking with purified GRP78 at physiologically relevant doses of either 4-HNE (B) or 4-ONE (C). Chemical stabilization of these adducts using NaBH4, displays the high susceptibility of this lysine cross-linking with concentrations as low as 3μM (1x).