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. Author manuscript; available in PMC: 2016 Mar 18.
Published in final edited form as: Clin Ther. 2015 Mar 18;37(3):540–551. doi: 10.1016/j.clinthera.2015.02.017

Table 1.

Salivary non-codingRNAs as disease-related molecular markers

Characterization
Study # Saliva fraction Disease Study cohort Technique Molecular profile
Weber et al., Clin. Chem. 2010 CFS characterization 5 healthy donors Human miScript Assay panel (Qiagen) – 714 miRNA miR-182*, miR-450b-5p, miR-622, miR-141, miR-26a, miR-145*, miR-135b*, miR-381, miR-96*, miR-1228, miR-431*
Michael et al., Oral Dis. 2010 exosomes characterization 2 healthy donors miRCURY LNA microRNA Array, v.10.0, (Exiqon, Denmark) let-7b, let-7c*, miR-128, miR-150*, miR-17, miR-1908, miR-212, miR-27b*, miR-29b, miR-29c, (Top-10)
Patel et al., Arch Oral Biol. 2011 WS characterization 20 healthy donors TaqMan1 Low Density Array Card (TLDA) Human miRNA Panel v2.0 (Applied Biosystems). miR-223, miR-191, miR-16, miR-203, and miR-24
Spielmann et al., Clin. Chem. 2012 CFS and WS characterization 8 healthy donors SOLiDTM Total RNA-Seq Kit and Barcoding Kit (modules 1–16) (Applied Biosystems) 224 snoRNAs
Gallo et al., PLoS One 2012 exosomes characterization Healthy donors (# N/A) TaqMan MicroRNA Assay, PN 4427975, Applied Biosystems miR-22, miR202, miR-203, miR-1273d
Ogawa et al., Biol. Pharm. Bull. 2013 exosomes and WS characterization 1 healthy donor (7 saliva collection replicates) Illumina Genome Analyzer Iix by Hokkaido System Sciences Co., Ltd. (Japan) miR-378a, miR-143, let-7c, miR-146b, miR-21, let-7f-1, let-7f-2, miR-30a, miR-9-1, miR-9-2, miR-9-3, let-7a-1, let-7a-2, miR-20a, miR-30d, miR-30e; piR-39980, piR-48209, piR-52207, piR-38581, piR-36095, piR-59293, piR-61648, piR-55361; U78, U44, U21, U31, U104, U15A, snR39B.
Bahn et al., Clin. Chem. 2014 CFS characterization 8 healthy donors Illumina HiSeq 50SE 127– 418 miRNAs (Top-2: miR-223–3p and miR-148a-3p)
Local & Systemic diseases
Study Saliva fraction Disease Study cohort Technique Molecular profile
Park NJ et al., Clin. Cancer Res. 2009 CFS and WS oral squamous cell carcinoma 50 OSCC patients and 50 healthy matched control subjects. RT-preamp-qPCR miR-125a and miR-200a
Wiklund et al., PLoS One 2011 WS oral squamous cell carcinoma 15 OSCC patients and 7 healthy control donors TaqManH qRT-PCR assays (Applied Biosystems) miR-375 and miR-200a expression and miR-200c-141 methylation
Liu CJ et al., Head Neck. 2012 CFS oral squamous cell carcinoma 45 oral carcinoma, 10 oral verrucous leukoplakia, and 24 healthy controls TaqMan miRNA assay system (Applied Biosystems, Foster City, CA) miR-31
Matse et al., Clin. Cancer Res. 2013 WS paratiroid gland tumors 38 malignant tumors and 29 benign parotid gland tumors TaqMan Human MicroRNA Cards (Applied Biosystems) and RTqPCR hsa-miR-132, hsa-miR-15b, mmu-miR-140, and hsa-miR-22
Tang et al., Mol. Med. Rep. 2013 SP oral squamous cell carcinoma 4 OSCC saliva samples and 12 healthy donors RT-qPCR of six lncRNAs found in OSCC tissue MALALT-1, HOTAIR
Wang et al., Biosens and Bioelectr, 2013 CFS oral squamous cell carcinoma 5 artificial saliva samples (spiked) Novel home-made electrochemical biosensor magnetic-controllable gold electrode miR- 200a, miR-142-3p, miR-93 and miR-125a
Xie Z et al., PLoS One 2013 CFS and WS esophageal squamous cell carcinoma NA Agilent miRNA microarray miR-144, miR-10b*, miR-21 and miR-451
Yang et al., BMC Cancer 2013 SP oral squamous cell carcinoma 7 non-progressing LGD, 8 progressing LGD into OSCC and 7 healthy control donors The TaqManW low density array (TLDA) qRT-PCR system (Applied Biosystems, Foster City CA) miR-10b, miR-660, miR-708, miR- 30e, miR-145, miR-99b, miR-181c and miR-197
Salazar et al., Cell Oncol. 2014 WS head and neck cancer 61 HNSCC patients and 61 healthy controls miScriptTM miRNA microarray, RTqPCR, TCGA miR-9, miR-134 and miR-191
Wang et al., Tumor Biol. 2014 2 saliva data sets, 6 plasma/serum data sets (meta-analysis) esophageal squamous cell carcinoma 995 ESCC patients and 733 healthy controls Bioinformatic and Statistics tools miR-144, miR-10, miR-451
Momen-Heravi et al., J Dent Res, 2014 CFS oral squamous cell carcinoma 9 OSCC patients before treatment, 8 patients with OSCC in remission, and 9 HCs. NanoString nCounter miRNA expression assay (NanoString Technologies, Seattle, WA, USA) miRNA-136, miRNA-147, miRNA-1250, miRNA-148a, miRNA- 632, miRNA-646, miRNA668, miRNA- 877, miRNA-503, miRNA-220a, miRNA-323-5p, miRNA-24, miRNA- 27b
Hizir et al., ACS appl. Mater. Interf. 2014 CFS prostate cancer NA nanographene oxide system miR-21, miR141
Forensic Science
Study # Saliva fraction Disease Study cohort Technique Molecular profile
Hanson et al., Anal. Biochem. 2009 WS body fluid identification Healthy donors (# NA) RTqPCR miR-658, miR-205
Zubakov et al., Int J Legal Med. 2010 WS body fluid identification Healthy donors (# NA) Microarray LNATM-modified oligo- nucleotides (Exiqon, Vedbæk, Denmark), and RTqPCR miR-583, miR-518c*, miR-208b
Courts et al., J Forensic Sci. 2011 WS body fluid identification Healthy donors (# NA) Microarray Geniom Biochips (Heidelberg, Germany) and RTqPCR miR-200c, miR-203, miR-205
Wang et al., Forensic Sci. Int: Gen, 2012 WS body fluid identification 10 healthy donors RTqPCR miR-658, miR-205
Omelia et al., Analyt Biochem, 2013 body fluid identification
Park JL et al., Electrophoresis 2014 WS body fluid identification 60 healthy donors Affymetrix Gene Chip miRNA 3.0 array and RTqPCR miR-203, miR-205
Silva et al., Forensic Sci. Int: Gen, 2015 – body fluid identification – – (Review)

LEGEND

WS: whole saliva

CFS: cell free saliva

SP: saliva pellet

NA: not avaliable