Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 13;209(1):33–46. doi: 10.1083/jcb.201408017

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Tsaalbi-Shtylik et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 6. — Epistasis of mutagenic TLS and Msh2/Msh6. (A) UVC (2 J/m²)-induced mutant frequencies in isogenic WT, Msh6^−/− (M), Rev1^B/B (R), and Msh6^−/−Rev1^B/B (MR) ES cells. Bars represent averages from three independent experiments. (B) Msh2/Msh6-dependent suppression of UVC-induced mutant frequencies in Rev1-proficient (TLS⁺) and Rev1^B/B (TLS⁻) ES cells, derived from A. Frequencies were calculated by subtraction of induced mutant frequencies in Rev1^B/B cells from those in Rev1^B/BMsh6^−/− cells in each individual experiment, followed by averaging. (C) In vivo assay to quantify mutagenic TLS at a site-specific (6–4)TT (triangle) on a transfected replicating plasmid. (D) Mutant frequencies at the 5′ and the 3′ thymidines of a site-specific (6–4)TT in NER-deficient (Xpc^−/−) mouse embryonic fibroblast lines in the presence (X) or absence (MX) of Msh2/Msh6. Bars represent averages from two experiments (see Fig. S5).