Figure 1.

SG proteins and polyadenylated mRNA are well recruited to SGs despite impaired SG aggregation. (A–C) HeLa cells were transfected with the indicated siRNAs for 72 h. Coverslips were collected at 90 min after SA treatment (0.5 mM, 30 min). (A) Representative Western blot showing the efficiency of siRNA transfections. (B) Coverslips were labeled for eIF4G, eIF3A, HuR, Caprin1, or USP10 and the SG marker TIA-1. Bar, 25 µm. (C) Polyadenylated mRNA was visualized via fluorescence in situ hybridization with an oligo(dT) probe and HuR and TIA-1 labeling. Bar, 10 µm. Representative micrographs from ≥3 independent experiments are shown. Line scans (from the white lines) were used to assess colocalization (histograms).