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. 2015 Apr 13;209(1):73–84. doi: 10.1083/jcb.201408092

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© 2015 Aulas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 5. — Decreased mRNA preservation after stress exposure. (A–C) Cells were transfected with the indicated siRNAs for 72 h and then subjected to two consecutive rounds of stress response induced by SA (SG assembly and disassembly). Coverslips were collected before stress, and after the first and the second round of SG assembly/disassembly. Polyadenylated mRNA was visualized via FISH with an oligo(dT) probe. (A) Experimental design. (B) Representative images of HeLa cells labeled with an oligo(dT) probe and quantification of total pixel intensity. (C) Representative images and quantification of a similar experiment performed in SH-SY5Y cells and examined only after the second SA stress exposure. All data are representative of >3 independent experiments and plotted as the mean ± SEM (error bars). *, P < 0.05. Bars, 10 µm.