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. 2015 Apr 13;209(1):23–32. doi: 10.1083/jcb.201407059

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© 2015 Juanes-Garcia et al.

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Figure 2. — Phosphomimetic mutation of the five Ser residues adjacent to the nonhelical domain of MHCII-B decreases NMII-B stability and front–back polarization. (A) MHCII-B–depleted CHO.K1 cells expressing the indicated shRNA-resistant GFP–MHCII-B mutants. Column on right depicts GFP. (top) Wild type; (middle) 5×A; (bottom) 5×D. Left column is F-actin. Note the lack of polarity in the cell rescued with the 5×D mutant. Representative examples are shown. Bars, 10 µm. (B) Polarity index of MHCII-B–depleted cells rescued or not rescued with the constructs shown in A. Data are the means ± SD of >200 cells from four independent experiments. (C) FRAP curves of the GFP–MHCII-B mutants used in A and B. Data are the means ± SEM of 24 individual measurements per condition in four independent experiments.