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. 2015 Apr 13;209(1):129–142. doi: 10.1083/jcb.201411087

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© 2015 Roberson et al.

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Figure 2. — Tmem231 mutant mice display MKS-like phenotypes. (A) Hematoxylin and eosin–stained sections of portal triads of E15.5 livers of wild-type and Tmem231^−/− embryos. In Tmem231^−/− embryos, the portal vein (P) and the bile duct (B) are surrounded by increased mesenchyme. Bar, 100 µm. (B) Hematoxylin and eosin–stained sections of E18.5 kidneys show cyst formation in Tmem231^−/− embryos. (C) Postnatal day 0 hindlimb skeletons stained with alcian blue and alizarin red reveal an extra digit (*) in Tmem231^−/− embryos. Bars, 1 mm.