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. 2015 Apr 13;209(1):129–142. doi: 10.1083/jcb.201411087

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© 2015 Roberson et al.

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Figure 3. — Tmem231 and B9d1 regulate ciliary membrane protein localization in vitro and in vivo. Tmem231 wild-type and mutant MEFs (A) and B9d1 wild-type and mutant MEFs (B) stained for γ-tubulin (γTub, red), acetylated tubulin (Tub^Ac, blue), and either Adcy3, Arl13b, Inpp5e, Pkd2, or Ift88 (green). Tmem231 and B9d1 are required for ciliary membrane protein localization, but not Ift88, a nonmembrane-associated ciliary protein. Bar, 2.5 µm. Quantitation of at least 10 cilia per condition are shown next to each figure. *, P < 0.05, as measured by Student’s t test with Welch’s correction, throughout. Error bars represent the 95% confidence interval, also throughout. B9d1 (C) and Tmem231 (D) wild-type and mutant tissues stained for Tub^Ac (green), Arl13b (red), and DAPI (blue). Insets are a magnification of the indicated portion. Bars: (full field) 10 µm; (inset) 2.5 µm.