Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Apr 13;209(1):129–142. doi: 10.1083/jcb.201411087

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Roberson et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 4. — Tmem231, B9d1, and Tctn1 are required for TZ MKS complex formation. Ciliary TZ of Tmem231 (A), B9d1 (B), and Tctn1 (C) wild-type and mutant MEFs, stained for γ-tubulin (red), Tub^Ac (blue), and either Tmem231, B9d1, Mks1, Tmem67, Rpgrip1l, or Nphp1 (green). Tmem231 and B9d1 are necessary for Mks1 and Tmem67 but not Rpgrip1L or Nphp1 localization. Tctn1 is required for both Tmem231 and B9d1 localization. TZs are denoted by white arrowheads. Bars, 2.5 µm. Quantitation of TZ fluorescence intensity from at least 10 cilia are shown next to each set of images. Error bars represent the 95% confidence interval. *, P < 0.05, as measured by Student’s t test with Welch’s correction. (D) Tmem231 wild-type and mutant bile duct, kidney, pancreas, retina, and trachea stained for B9d1 (green), Tub^Ac (red), and DAPI (blue). (E) B9d1 wild-type and mutant bile duct, kidney, pancreas, retina, and trachea stained for Tmem231 (green), Tub^Ac (red), and DAPI (blue). Bars: (full field) 10 µm; (inset) 2.5 µm.