A. Compared to controls, patients with NAFLD and NASH had higher hepatic sterol regulatory element binding protein-1 (SREBP-1) expression (P<.03 NAFLD/NASH vs control). Laminin was used as a loading control.
B. Rate of 3H-acetate and 14C-palmitate incorporation into triglycerides by hepatocytes was increased in NAFLD/NASH (P<.01) and decreased with progression to cirrhosis (P<.01 NAFLD/NASH vs cirrhosis).
C. Rate of 3H-acetate into cholesterol in hepatocytes was significantly higher in NAFLD/NASH (P<.05 vs controls) and trended down with progression to cirrhosis (p=0.07 NAFLD/NASH vs cirrhosis).
D. Desmosterol:total cholesterol ratio was significantly higher in subjects with NAFLD and NASH compared to lean and obese controls (P<.02 lean/obese controls vs. NAFLD/NASH). All data is presented as means ± S.E.M.