Fig. 4. Effect of manganese instillation and Hfe deficiency on the expression of nicotinic acetylcholine receptor in prefrontal cortex and hippocampus. Prefrontal cortex (A) and hippocampus (B) collected from mice intranasally instilled with MnCl2 were homogenized to determine the expression levels of α7-nicotinic acetylcholine receptor (nAChR). Relative intensities of protein bands normalized to actin were determined using Image Lab (version 4.1). Empty and closed bars represent water-instilled and MnCl2-instilled mice, respectively. Data were presented as means ± SEM (n = 4 per group) and were analyzed using two-way ANOVA.
