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. 2015 Jan 20;23(4):737–745. doi: 10.1038/mt.2014.242

Figure 2.

Figure 2

Perforin reconstitution in peripheral NK and T cells leads to restoration of cytotoxic function. NK and CD8+ T cells from prf−/− mice were transduced with PGK.GFP, PGK.PRF, or with PRF.PRF vectors and processed for chromium assays. (a) 51Cr release from RMA-S cells co-incubated with transduced and untransduced NK cells and WT mice NK cells. (b) 51Cr release from anti-CD3 bound P815 cells co-incubated with transduced and untransduced CD8+ T cells and WT mice CD8+ T cells. The effector to target ratios were normalized taking into consideration the percentage of GFP-positive effector cells (7% in NK cells and 10% in CD8+ T cells with both PRF vectors). The P values correspond to both the comparisons between the PGK.PRF and the PRF.PRF groups with the prf−/− group. The results presented show one representative experiment from a series of three experiments, and the error bars represent the SEM from the chromium assay triplicates.