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. 2015 Jan 20;23(4):737–745. doi: 10.1038/mt.2014.242

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Copyright © 2015 The American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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Efficient lentiviral vector transduction of progenitor cells and demonstration of normal colony formation from transduced cells. (a) Flow cytometry plots showing GFP expression in LSK cells transduced with PGK.GFP, PGK.PRF, or PRF.PRF. (b) Transduction efficiency and viral copy number of the LSK cells transduced with PGK.GFP, PGK.PRF, and PRF.PRF. (c) The same cells were used in a hematopoietic colony formation assay: BFU-E, burst forming unit erythroid; CFU, colony forming unit granulocyte macrophage; CFU-GEMM, colony forming unit granulocyte, erythroid, macrophage, megakaryocyte. The results presented show one representative experiment from a series of three experiments, and the error bars represent the SEM from the colony formation assay triplicates.