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. 2015 Jan 20;23(4):656–666. doi: 10.1038/mt.2014.247

Figure 5.

Figure 5

miR-125a-5p is repressed by HDAC5. R2N1d cells were transfected with the indicated amounts of an HDAC5 expression or silencing plasmid (μg/ml). At 24 hours post-transfection, miR-125a-5p expression was detected using western blot and qRT-PCR (a–d), and the cell growth rate was evaluated by determining XTT assay (e). The control miRNA (5 μg/ml), miR-125a-5p (5 μg/ml), HDAC5 (5 μg/ml), or miR-125a-5p (5 μg/ml) + HDAC5 (5 μg/ml) were transfected into R2N1d cells and the cells assessed at 48 hours post-transfection. (f) Apoptosis was evaluated with the TUNEL assay and the wound healing rates were evaluated (g). Values are the mean ± SD of three experiments. *P < 0.05 versus untreated control; Two-tailed Student's t-test.