Figure 3.

CM-hfMSCs induced cell cycle arrest of hepatocellular carcinoma (HCC) cells. (a) Immunocytochemistry staining of CM-treated-Huh7-DsRed2 cells against human Ki-67. Photomicrographs showed representative field images of Ki-67-stained cells. Scale bar = 100 µm. Bar graph represents percentage of Ki-67-positive cells (scored from 16 random field of view) normalized to CM-Ctrl-treated cells, n = 16. (b) Flow cytometry analysis of the cell cycle profile of CM-treated Huh7-DsRed2 cells. Representative diagrams were shown. (c) Immunoblot analysis of cell cycle–related proteins from CM-treated-Huh7-DsRed2 cell lysates. Protein loaded was normalized to actin. (d) Ki-67 and (e) cyclin D1 immunohistochemistry staining of cryosectioned tumor harvested from CM-Ctrl, CM-MRC5, and CM-S8-treated patient-derived tumor 26–1004. Photomicrographs showed representative images. Scale bar = 50 μm. Immunopositive cells were quantified (total 10 random field of view) and presented in the bar charts. Data are shown as means ± SEM, n = 10; **P < 0.01.