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Size exclusion chromatography of bispecific antibodies in two different formats. Two FLT3 X CD3-antibodies with identical specificities (4G8 X UCHT1) in the Fabsc- (a) and bssc-format (b) were analyzed on a Superdex 200 PC3.2/30 column. Approximately 70% of the bssc-material consists of dimers and higher molecular weight aggregates. This percentage was found to be approximately 35, 50, and 70% respectively, in three additional, independently produced batches.
