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β-actin::GFP-Smad2 transgene rescues smad2/smad2 adult lethality
| smad2/+ X smad2/+; Tg(GFP-Smad2)/+ | ||
|---|---|---|
| Genotype | +/+ | Tg(gfp-smad2)/+ |
| +/+ | 11 (37%) | 4 (15%) |
| smad2/+ | 19 (63%) | 17 (60%) |
| smad2/smad2 | 0 (0%) | 7 (25%) |
smad2/+ fish were crossed to smad2/+; Tg(GFP-Smad2)/+ fish and their progeny was raised to adulthood and genotyped for smad2 and for Tg(GFP-Smad2). The only recovered adult progeny homozygous for smad2 contains a copy of the GFP-Smad2 transgene.
