Table 1.
GeXP primers sequence and amplicon sizes designed for quantification of chicken cytokines, chemokines and other immune-related genes
| Gene | Accession number | Amplicon size (bp) | Forward primer sequence* (5′–3′) | Reverse primer sequence** (5′–3′) |
|---|---|---|---|---|
| Cytokines and chemokines | ||||
| CCL4 | NM_204720 | 235 | CTGCTCAAAGCCTGCCATC | GTGCAGCCATCCTGAAGC |
| CXCLi1 | NM_205018 | 228 | CCGATGCCAGTGCATAGAG | CCTTGTCCAGAATTGCCTTG |
| CXCLi2 | NM_205498 | 165 | CCTGGTTTCAGCTGCTCTGT | GCGTCAGCTTCACATCTTGA |
| GM-CSF | NM_001007078 | 242 | TGAAAACAAATGGGACAGAGG | TTCTCCTCTGGGAGCACATC |
| IFN-γ | NM_205149 | 214 | GAGCCATCACCAAGAAGATGA | TAGGTCCACCGTCAGCTACA |
| IL-1β | NM_204524 | 137 | CCAGAAAGTGAGGCTCAACA | GTAGCCCTTGATGCCCAGT |
| IL-2 | NM_204153 | 144 | GTGGCTAACTAATCTGCTGTCCA | CCGTAGGGCTTACAGAAAGG |
| IL-4 | NM_001007079 | 151 | CGTCAAGATGAACGTGACAGA | AGGTTCTTGTGGCAGTGCT |
| IL-6 | NM_204628 | 158 | AGTTCACCGTGTGCGAGAAC | TTCGTCAGGCATTTCTCCTC |
| IL-10 | NM_001004414 | 172 | TAACATCCAACTGCTCAGCTC | TGATGACTGGTGCTGGTCTG |
| IL-12α | NM_213588 | 179 | AAGGGACTCAACTGCTCCAG | TTGTGTTGCTCTGACTGTTGG |
| IL-15 | NM_204571 | 193 | ATTCCCGATCCAGATTCTGTT | ACAGTTGGTACTGGAGACAAATACT |
| IL-16 | NM_204352 | 200 | GCCTCACAAGAATCAACAACTG | TGCTTTGTTCCAACGAGGTC |
| IL-17 F | NM_204460 | 340 | TCCATGGGATTACAGGATCG | AGGCAAGGCAGTTCTCCTG |
| IL-18 | NM_204608 | 207 | CGTCAATAGCCAGTTGCTTG | CTTCTACCTGGACGCTGAATG |
| IL-21 | NM_001024835 | 186 | TGTGGTGAAAGATAAGGATGTCG | CAGTTTTGGCGAATGTAGCA |
| IL-22 | XM_416079 | 256 | CAGCCCTACATCAGGAATCG | GAACTGTGCCACATCCTCAG |
| TGF-β3 | NM_205454 | 347 | AATCAGCATACACTGCCCTTG | TCGGAAGTCAATGTAAAGAGGAC |
| TNFSF13B | NM_204327 | 333 | GGCAAGGTCTCCACTAGAGC | TCAGAAGCCAAGGGACAATG |
| Toll-like receptors | ||||
| TLR2-1 | NM_204278 | 249 | TCAGCTACACCAAAATGTTCAACC | CGTGATTTTGCCTGTGAGC |
| TLR3 | NM_001011691 | 263 | TGCATAAGAAGGAGCAGGAAG | CTGGCCAGTTCAAGATGCAG |
| TLR4 | NM_001030693 | 270 | CATCTCTGGAGTTCCTGCTG | AGGCTGCTAGACCCAGGTG |
| TLR5 | NM_001024586 | 277 | CACTCAGGTTCTCGGTATTCG | AATCCAGGTGCTTCAGCAAG |
| TLR7 | NM_001011688 | 284 | GAGTGAGTTATGCCACTCCTCTC | TCAAAGGCTTCCACATCAC |
| Others | ||||
| iNOS | NM_204961 | 221 | TATGCTCTGCCTGCTGTTGC | ATGCAAGTTTGTTGCTTTCC |
| MHCI | NM_001044683 | 291 | GGAAACCTGCGTGGAGTG | TGGTGACCCAGGTGTGGTA |
| MHCII | NM_001044679 | 298 | AGTACGCGCACTTCGACA | AGAAGCCCGTCACGTAGC |
| GAPDH | NM_204305 | 312 | CTGGCAAAGTCCAAGTGGTG | AGCACCACCCTTCAGATGAG |
| KAN r*** | - | 325 | ATCATCAGCATTGCATTCGATTCCTGTTTG | ATTCCGACTCGTCCAACATC |
*Forward universal primer sequence (AGGTGACACTATAGAATA).
**Reverse universal primer sequence (GTACGACTCACTATAGGGA).
***Internal control.