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Effect of rapamycin on auditory HCs by cochlear turns. Photograph of anti-myosin VIIa-labeled OCs. Scale bar 20 μm. (a–f) Untreated OCs (control) and OCs exposed to 10 μM rapamycin demonstrate three rows of OHCs and a single row of IHCs in all cochlear turns. (g–l) OCs treated with increasing concentrations of rapamycin (50 μM and 100 μM, resp.) show a dose dependent loss of OHCs and IHCs.
