Skip to main content
. 2015 Mar 30;2015:304753. doi: 10.1155/2015/304753

Figure 1.

Figure 1

dsP21-397 induces p21 expression by targeting a complementary promoter sequence in BC cells. (a) Schematic representation of the p21 promoter and dsP21-397 target site. Sequence of the miR-1180-5p target site located at nucleotide -397 relative to the transcription start site. (b and c) Expression of p21 and GAPDH mRNA levels was assessed by real-time PCR. GAPDH served as a loading control. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs for 72 h. mock sample was transfected in the absence of dsRNA. ∗ P < 0.001 compared to mock and dsControl groups. (d) Induction of p21 protein expression was detected by Western blot analysis. GAPDH levels were also detected and served as a loading control.