Table 4.
Top 10 upregulated and top 10 downregulated genes in CD133+ relative to CD133- HCT116 cells
| A. Top 10 Upregulated Genes | |||
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| Gene Symbol | Gene Name | Log Fold Change | P-Value |
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| PROM1 | prominin 1 | 2.8 | 0.0106 |
| EHF | ets homologous factor | 2.3 | 0.0106 |
| NRIP1 | nuclear receptor interacting protein 1 | 2.2 | 0.0114 |
| GPR110 | G protein-coupled receptor 110 | 1.7 | 0.0188 |
| IL18 | interleukin 18 (interferon-gamma-inducing factor) | 1.6 | 0.0114 |
| DPP4 | dipeptidyl-peptidase 4 (CD26, adenosine deaminase complexing protein 2) | 1.4 | 0.0114 |
| SEMA3A | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A | 1.4 | 0.0114 |
| 7A5 | putative binding protein 7a5 | 1.4 | 0.0114 |
| LOC390345 | similar to ribosomal protein L10 | 1.3 | 0.0188 |
| SNORA22 | small nucleolar RNA, H/ACA box 22 | 1.3 | 0.0396 |
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| B. Top 10 Downregulated Genes | |||
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| Gene Symbol | Gene Name | Log Fold Change | P-Value |
|
| |||
| SLC2A3 | solute carrier family 2 (facilitated glucose transporter), member 3 | -1.7 | 0.0167 |
| ODZ3 | odz, odd Oz/ten-m homolog 3 (Drosophila) | -1.7 | 0.0106 |
| PEG10 | paternally expressed 10 | -1.5 | 0.0114 |
| MAP1B | microtubule-associated protein 1B | -1.4 | 0.0106 |
| GLIS3 | GLIS family zinc finger 3 | -1.4 | 0.0188 |
| CTGF | connective tissue growth factor | -1.3 | 0.0352 |
| THBS1 | thrombospondin 1 | -1.3 | 0.0210 |
| SEMA3C | sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C | -1.2 | 0.0377 |
| RBM24 | RNA binding motif protein 24 | -1.2 | 0.0210 |
| CDK6 | cyclin-dependent kinase 6 | -1.1 | 0.0114 |
Expression of mRNA levels in CD133+ cells compared to CD133- cells (methods and details are described in the text). Gene symbols and gene names (according to the Affymetrix hugene1.0-chip) are given as well as the log-fold change [log(mean group CD133+) minus log(mean group CD133-)] and the corresponding P-value of the t-test (adjusted for multiplicity using the method of Benjamini and Hochberg) of the top 10 up- and downregulated genes. A P-value <0.05 is indicating a significant difference between CD133+ and CD133- cells.