Table 5.
Comparative pathway analysis of expression of growth regulatory genes in CD133+ as opposed to CD133- HCT116 cells
| Gene Symbol | Gene Name | Mean CD133+ | Mean CD133- | StDev CD133+ | StDev CD133- | P-Value |
|---|---|---|---|---|---|---|
| ACTL6A | actin-like 6A | 8.2 | 8.1 | 0.3 | 0.4 | 0.6729 |
| CAMK2D | calcium/calmodulin-dependent protein kinase (CaM kinase) II delta | 7.9 | 8.4 | 0.2 | 0.3 | 0.1264 |
| CTGF | connective tissue growth factor | 7.0 | 8.3 | 0.1 | 0.6 | 0.0622 |
| IGFBP4 | insulin-like growth factor binding protein 4 | 6.4 | 6.4 | 0.2 | 0.3 | 0.8422 |
| IGFBP6 | insulin-like growth factor binding protein 6 | 8.3 | 8.8 | 0.2 | 0.3 | 0.0894 |
| CYR61 | cysteine-rich, angiogenic inducer, 61 | 8.5 | 9.5 | 0.1 | 0.2 | 0.0059 |
| QSOX1 | quiescin Q6 sulfhydryl oxidase 1 | 8.3 | 8.2 | 0.5 | 0.4 | 0.7980 |
| SHC1 | SHC (Src homology 2 domain containing) transforming protein 1 | 8.7 | 9.0 | 0.1 | 0.1 | 0.0124 |
| YEATS4 | YEATS domain containing 4 | 8.2 | 7.9 | 0.3 | 0.4 | 0.4214 |
| LTBP4 | latent transforming growth factor beta binding protein 4 | 8.0 | 8.1 | 0.4 | 0.4 | 0.7153 |
| BLZF1 | basic leucine zipper nuclear factor 1 (JEM-1) | 8.0 | 7.9 | 0.3 | 0.3 | 0.8172 |
| RUVBL1 | RuvB-like 1 (E. coli) | 9.1 | 8.8 | 0.3 | 0.4 | 0.4854 |
| SOCS1 | suppressor of cytokine signaling 1 | 6.8 | 6.7 | 0.1 | 0.3 | 0.4293 |
| SOCS2 | suppressor of cytokine signaling 2 | 6.7 | 6.6 | 0.2 | 0.3 | 0.4856 |
| ENOX2 | ecto-NOX disulfide-thiol exchanger 2 | 6.5 | 6.3 | 0.3 | 0.3 | 0.5228 |
| BRD8 | bromodomain containing 8 | 7.2 | 7.5 | 0.2 | 0.4 | 0.2988 |
| TMEM97 | transmembrane protein 97 | 9.3 | 9.5 | 0.2 | 0.3 | 0.6351 |
| CRIM1 | cysteine rich transmembrane BMP regulator 1 (chordin-like) | 8.5 | 9.3 | 0.2 | 0.4 | 0.0360 |
| ING3 | inhibitor of growth family, member 3 | 7.0 | 7.0 | 0.3 | 0.4 | 0.9326 |
| SOCS4 | suppressor of cytokine signaling 4 | 6.6 | 6.4 | 0.3 | 0.5 | 0.5587 |
| BTG1 | B-cell translocation gene 1, anti-proliferative | 8.4 | 8.7 | 0.4 | 0.3 | 0.4571 |
| PPP2CA | protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform | 9.9 | 9.9 | 0.3 | 0.3 | 0.8012 |
| PPP2R1B | protein phosphatase 2 (formerly 2A), regulatory subunit A, beta isoform | 8.2 | 8.2 | 0.2 | 0.2 | 0.9676 |
| RB1 | retinoblastoma 1 (including osteosarcoma) | 8.0 | 8.2 | 0.3 | 0.3 | 0.5369 |
Expression of mRNA levels in CD133+ cells compared to CD133- cells (methods and details are described in the text). Gene symbols and gene names (according to the Affymetrix human gene1.0 chip) are given as well as the mean values of the CD133+ and CD133- cells and the corresponding standard deviations (StDev). Furthermore, the P-values of the t-test to compare CD133+ to CD133- cells for each gene are shown. A P-value <0.05 indicates a significant difference between CD133+ and CD133- cells.