Figure 2.

Secretory expression of SPRY1 and SPRY2 by S. typhimurium strain VNP20009. A. Schematic diagram of the construction of vectors for expression of SPRY1 and SPRY2 in VNP20009. B. The indicated stable strains were grew in anaerobic or aerobic jars and their bacteria lysates and culture supernatant were examined for the expression of genes of interest through western blotting as described in the methods. C. The relative tissue distribution of indicated stable strains were detected by colony formation assay. *p < 0.001, tumor compared with the other tissues. Data are expressed as mean ± SD of three animals. D. Detection of the expression of genes of interest in the liver, spleen, lung, kidney, and tumor tissues of the mice bearing melanoma by western blotting. α-tubulin was served as loading control. One representative of three independent experiments is displayed.