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. 2015 Jan 15;5(2):481–497.

Figure 3.

Figure 3

Fisetin induced nitric oxide causes DNA double strand breaks. A. Western blots of fisetin (20 µM for 6 h) treated cells in the presence (Group 4) or absence (Group 2) of NO inhibitor, cPTIO shows that scavenging of NO prevents fisetin induced double strand breaks. Etoposide (20 μM for 18 h) (Group 3) was used as a positive control and actin as the loading control. All western blots are representative of a minimum of 3 repeats. Bar graph shows relative expression of gamma H2A-X phospho S139. Data are ± SEM, n = 3, *p < 0.05. B. Number of cells positive for double strand break was measured using fluorescence microscopy. Groups are 1, control; 2, fisetin; 3, etoposide; 4, fisetin + cPTIO; 5, etoposide + cPTIO; 6, fisetin + cPTIO + etoposide. Data are ± SEM, n = 3. 150-200 cells were analyzed per assay. C. Representative microphotographs showing cells stained for DNA double strand breaks under different conditions.