Figure 6.

DTWD1 functions as a tumor suppressor by regulating cyclin B1. The effect of ectopic expression of DTWD1 (A) on cell growth was determined by colony formation assay (B) and growth curve assay (C). Asterisks indicate statistical significance (p < 0.05). The effect of DTWD1 expression on the growth of gastric cancer cells in vivo was analyzed by inoculating SGC7901 cells into nude mice. The growth of tumors and immunohistochemistry staining of mice tumors were shown in (D) (student’s t-test, P < 0.05) and (E). The effect of DOX-induced DTWD1 expression on expression of cyclin B1, p21, CDK6, cyclin D1, cyclin A2 and cyclin H in DOX-induced DTWD1 SGC7901 cells were determined by Western blotting (F).