Table 1.
Name | Sequence |
---|---|
Primers | |
DTWD1 cloning | F: GGGATCCTCTCTCAATCCACCTAT |
R: GCTCGAGCTAATGTGTAAGTTTTCCTGTTTCC | |
DTWD1 RT-PCR | F: ATTCACTGATGAGCGATTC |
R: ATCAGTATGGTAGTCTACCAG | |
GAPDH RT-PCR | F: GGAGTCAACGGATTTGGT |
R: GTGATGGGATTTCCATTGAT | |
DTWD1 ChIP PCR | F: TTCCAGTTGGCTAAGACT |
R: AACAACCCTCCCACTCTT | |
DTWD1 promoter cloning | F1: GGAGCTCCTATTCTACTTTCCCTACCC |
R1: AAGCTTGGAACACTATACAGACTCCC | |
F2: GGAGCTCCTCTGGAGACCAGTGGTC | |
F3: GGAGCTCTTTCGAGAACCGCTTCCC | |
R2: GAAGCTTCTGACAAAGAATGTCCAC | |
siRNAs | |
HDAC1 | CCGGUCAUGUCCAAAGUAATT |
UUACUUUGGACAUGACCGGTT | |
HDAC2 | CCAGAACACUCCAGAAUAUTT |
AUAUUCUGGAGUGUUCUGGTT | |
HDAC3 | GAGCUUCCCUAUAGUGAAUTT |
AUUCACUAUAGGGAAGCUCTT | |
HDAC8 | GUCCCGGUUUAUAUCUAUATT |
UAUAGAUAUAAACCGGGACTT | |
p53 | CCACCAUCCACUACAACUATT |
UAGUUGUAGUGGAUGGUGGTT |