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. 2015 Jan 15;5(2):514–529.

Figure 3.

Figure 3

Podoplanin meditates the actin cytoskeleton remodeling through RhoA/Cdc42/Rac1 pathway in OSCC cells. A. Cell spreading assays with extracellular matrix (collagen I and Fibronectin) were performed in all the transfected OSCC cells, and then the transfected OSCC cells were stained with fluorescein isothiocyanate (FITC)-conjugated phalloidin to reveal filamentous actin stress fibres (green). Scale bar = 100 μm (phase); Scale bar = 100 μm (immunofluorescence). B. The activities and expressions of Rho family small GTPases were analyzed by Rho family pull-down assays in Podoplanin-transfected OSCC cells. C. Podoplanin-transfected OSCC cells were treated with small molecule inhibitors of Rho-associated kinase (Y27632), and then the activities and expressions of Rho family small GTPases were analyzed by Rho family pull-down assays. Experiments in A-C were performed in triplicates (n = 3); error bars indicate SD; *P < 0.05 and **P < 0.01 vs. Mock group; #P < 0.05 and ##P < 0.01 vs. si-con group.