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. 2015 Jan 15;5(2):545–559.

Figure 4.

Figure 4

miR-100 regulated the radiosensitivity of CRC cells CCL-244. A. Quantitative real-time PCR was used to confirm the transfection efficiency. U6 RNA was used as an internal control. The mean ΔCt of miR-100 in CCL-244 cells treated with miR-100 mimics and negative control miR-100 mimics (*P < 0.05). B. CCL-244 cells treated with miR-100 mimics and negative control miR-100 mimics. After 24 h, the cells were subjected to 8-Gy irradiation. After 48 h, CCK-8 was used to detect cell viabilities (miR-100 mimics compared with miR-100 negative control, *P < 0.05 or untransfected, **P < 0.01). These results are representative of at least three separate experiments. C. CCL-244 cells treated with miR-100 mimics and negative control miR-100 mimics. After 24 h, the cells were subjected to 0, 2, 4, 6 and 8 Gy of irradiation. After 14 days, the colony formation rate in different treatment groups was examined.