Skip to main content
. 2015 Jan 15;5(2):545–559.

Figure 5.

Figure 5

miR-100 promoted X-ray-induced apoptosis of CCL-244 cells. A. Flow cytometry assay to determine the apoptosis of CCL-244 cells transfected with miR-100 mimics or negative control miR-100 mimics and non-transfected cells. Percentage of apoptotic cells in non-transfected and transfected cells subjected to 0 and 8-Gy irradiation. Data are means ± SEM of three independent experiments. miR-100 significantly increased the X-ray-induced apoptosis of CCL-244 cells compared with non-transfected (**P < 0.01) and negative control miR-100 mimics (*P < 0.05) as well as the apoptosis of nonirradiated CCL-244 cells compared with non-transfected (*P < 0.05) and negative control miR-100 mimics (*P < 0.05). B. Western blot analysis of apoptotic marker proteins p53, Caspase-3, Bcl-2 or NF-κB in CCL-244 cells with miR-100 in transfected and non-transfected cells before and after exposure to 8-Gy X-ray irradiation. β-Actin was used as an internal control.