Skip to main content
. 2015 Jan 15;5(2):714–725.

Figure 4.

Figure 4

ROS are required for HMGB1 translocation and enhanced autophagy. A. NB4 cells were pretreated NAC (2 mM) or SOD1 RNAi and then treated with ATRA (1 μM) for 72 h. And the level of LC3-I/II was assayed by Western blot. UT, untreated group. B. NB4 cells were pretreated with NAC (2 mM) or SOD1 RNAi and then ATRA (1 μM) for 72 h. And the nuclear/cytosolic HMGB1 expression was assayed by Western blot; C. NB4 cells were treated with ATRA (1 μM) for 72 h with or without NAC (2 mM) pretreatment and then immunostained with HMGB1-specifc antibody (red) and Hoechst 33342 (blue) (400 × magnification). D. NB4 cells were pretreated with NAC (2 mM) or SOD1 RNAi and then ATRA (1 μM) for 24-72 h. And the release of HMGB1 was analyzed by ELISA. Cell viability of control was set as 100%. *P < 0.05, #P < 0.05 (n = 4, Student’s t-test).