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. 2015 Apr 11;15:25. doi: 10.1186/s12896-015-0139-7

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© Shinozuka et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MspJI-enzymatic digestion of 5^mC-containing PCR and Φ29 products. (a) DNA fragments amplified with the locus-specific PCR primers for the Agro_gc50 sequence under the presence of 5-methyl-dCTP (0, 2, 4 or 8 μM). (b) MspJI-digested DNA fragments derived from PCR products with each locus-specific primers and Agro gDNA as DNA template. Molar concentration denotes the 5-methyl-dCTP-concentration in PCR solution. (c) MspJI-enzymatic digestion of Φ29 enzyme-amplified DNA with randomly incorporated 5^mC from a range of DNA templates. 0, 10, 15 and 20 μM denote final concentrations of 5-methyl-dCTP in the REPLI-g WGA mixture.