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. 2015 Apr 11;15:25. doi: 10.1186/s12896-015-0139-7

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© Shinozuka et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Sequencing of the fungal endophyte genome with the MspJI-based DNA fragmentation method. Two independent experiments (rep1 and rep2) were performed using the single endophyte strain. (a) WGA of the endophyte genome with the Φ29 enzyme in the presence of 5-methyl-dCTP (X μM). (b) MspJI digestion of the endophyte genome-derived amplicons. (c) Flowchart of the MspJI- and physical shearing-based library prep procedures from WGA products.