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T7E1 mutation detection assays for the cleavage activity of the Cas9/I2-1 system in 293T cells. (a) On-target cleavage activities of the Cas9/I2-1 system by different concentrations. The mutated rate is quantified by ImageJ. (b) Off-target cleavage activities of the Cas9/I2-1 system (n = 3).
