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. 2015 Mar 12;6(1):23. doi: 10.1186/s13287-015-0009-1

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© Bobba et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Cell growth from limbal and conjunctival biopsies. Phase-contrast images of limbal (A, C, D) and conjunctival (B, E) biopsies excised from patients with limbal stem cell deficiency and cultured over a specific period (see panel label for case identification number and time in culture). Although cultures displayed ample proliferation activity, some grafts failed (A-C) whereas others were successful (D and E) at last follow-up. A representative polymerase chain reaction for mycoplasma (F) on conditioned media derived from cultured cells from patient 12 (S2) is shown. S1 (positive control) shows a band at 259 base pairs (bp), and S3 is a negative control.