Figure 1.

Morphology and phenotypic characterization of BMSCs.
(A) Morphology of BMSCs at passage 3 (inverted phase contrast microscope, × 100; scale bar: 500 μm). (B) BMSC nuclei appeared blue (DAPI), and the cytoplasm appeared red (CM-DI) (fluorescence microscope; immunofluorescence staining, × 100; scale bar: 500 μm). (C) Flow cytometric analysis of BMSCs labeled with CD29 (positive), CD34 (negative), CD44 (positive), and CD45 (negative) antibodies. BMSCs: Bone marrow mesenchymal stem cells; DAPI: 4′,6-diamidino-2-phenylindole.