Figure 4.

Changes in antioxidant-like protein-1 (AOP-1) immunoreactivity in the hippocampal dentate gyrus of rats after strep tozotocin (STZ) treatment.

No obvious change in AOP-1 immunoreactivity was observed in rats after STZ treatment. (A–D) Photomicrographs of immunohistochemistry for AOP- 1 immunoreactivity in the hippocampal dentate gyrus in controls (A) and rats treated with STZ for 2 (B), 3 (C) and 4 weeks (D). AOP-1 immunoreactivity is found in interneurons in the polymorphic layer (PoL) of the dentate gyrus. ML: Molecular layer; GCL: granule cell layer. Scale bar: 100 μm. (E) Number of AOP-1-immunoreactive cells per section of each group. Differences between groups were analyzed statistically by one-way analysis of variance followed by Bonferroni's post-hoc analysis (n = 5 per group). There are no significant differences in the number of AOP-1 immunoreactive cells between groups. The bars indicate the standard error of the mean (SEM). STZ-2W, STZ-3W, STZ- 4W mean treatment with STZ for 2, 3 and 4 weeks, respectively.