Figure 1.

Isolation and preparation of equine ASCs in the cell culture lab. ASCs are isolated and prepared according the following procedure: A. Surgical resection of adipose tissue from the region of the horse’s tail. B. Adipose tissue dissolved in PBS in a Falcon tube. C. Mechanical disintegration of fat in the cell culture dish. D. Enzymatic digestion using collagenase I. E. After centrifugation the floating fat tissue is separated from the stromal vascular fraction at the bottom of the tube. F. After resuspension of the pellet in buffer (PBS) the suspension is filtered through a 70 µm nylon cell strainer and centrifuged again. G. The resulting pellet after the second centrifugation step. H. After resuspension in the standard medium the cells are plated in cell culture flasks or petri dishes.