Figure 2.

Selection of dissociation buffers for single cell suspension. A. Approximately 1 × 10⁶ hPSCs (KhES-1) cultured in TesR-E8 medium on rhVTN-N-coated dish were dissociated into single cells with the following dissociation reagents: Accutase, x1 TrypLE Select, Pronase, and 0.5 mM EDTA/PBS(-). The percentage of cells that were viable after dissociation is shown as a bar (means) with error bars (SD: Standard Deviation) (n = 5). **p = 0.0112 < 0.05. B. The percentage of cells that were viable after treatment with a cocktail consisting of TrypLE Select and 0.5 mM EDTA/PBS(-) at ratios of 1:0 (x1 TrypLE Select), 0.75:0.25 (x0.75), 0.5:0.5 (x0.5), and 0.25:0.75 (x0.25) is shown in bar graphs with error bars (n = 5). C. Time-lapse photographs of dissociation of KhES-1 cells on rhVTN-N-coated dishes treated with x0.75 TrypLE Select. Scale bar = 200 μm.