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A and B. 50 μM Neogrifolin treatment for 24 h induced activation of caspase-9, caspase-3, and cleavage of PARP in osteosarcoma cells. z-VAD-fmk, a universal inhibitor of caspases, prevented caspase-3 activation and PARP cleavage. C. Cell growth inhibition induced by neogrifolin was abolished by z-VAD-fmk preincubation in osteosarcoma cells.
