Figure 3.
Colocalization and internalization of CD63 and CD4 in human MDMs and U373-MAGI-CCR5 cells. After blocking with non-specific antibodies, A. MDMs (5 × 105 cells/well) and (B) U373 cells (5 × 105 cells/well) were incubated with anti-CD63 antibody (Caltag, labeled by Alexa 568) and/or anti-CD4 (Becton-Dickinson, labeled by Alexa 488). For time 0 min, cells were fixed with 4% paraformaldehyde and then stained with Alexa-labeled antibodies for 30 min at RT, and then fixed for an additional 20 min. For all other samples, cells were incubated with antibody for the indicated times at 37°C, and then washed twice in cold PBS and fixed with 4% paraformaldehyde.