Figure 4.
Virus Env-mediated entry in CD63-silenced human MDMs. MDMs (5 × 105 cells/well) were transfected with 50 nM siRNAs (CD63, CD4, and ERBB2IP). Forty eight hours post-transfection, cells were infected with various Env-specific pseudoviruses (m.o.i. = 0.02). Cell lysates were collected on day 3 post infection, and luciferase activity was analyzed to monitor pseudovirus integration. MLV and VSV were used as controls because they enter the cell via endocytosis. ERBB2IP siRNA was used as a cellular target negative control because ERBB2IP does not inhibit HIV-1 {Murray 2005}. CD4 siRNA was used as a control because it is the main receptor for entry of HIV. R5 (ADA) & dual-tropic (89.6) viruses were used because MDMs are R5-tropic and uses the main receptor CD4 and CCR5 co-receptor to enter the cell. All experiments were performed in quadruplicate. *P < 0.05, **P < 0.01, compared with ERBB2IP group.