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CD63 presents in macrophages produced HIV-1 virions. HIV-1 virions produced by macrophages and PBLs were concentrated. HIV-1 viral proteins were separated by electrophoresis, then transferred to a membrane which was incubated with (A) HIV-1 IgG, and (B) anti-CD63, followed by incubating with an enzyme- labelled antibody. A substrate was added for visualization. Lane 1, 2, 5, 6 were loaded with macrophage produced virions, and lane 3, 4, 7, 8 were PBLs produced virions.
