Effects of dihydroartemisinin on mRNA expression of TGF-β1, TNF-α, α-SMA, and NF-κB. A. Representative images of significant differences among groups are indicated by RT-PCR. B. Quantitative analysis of TGF-β1, TNF-α, α-SMA, and NF-κB. Averages of at least six independent experiments (n = 6). Bars indicate the mean ± SD. β-actin was used as internal control. Statistical analysis was performed using one-way ANOVA followed by multiple comparison test (*P < 0.05).